Department of Biological Sciences
Indian Institute of Science Education and Research Bhopal


Personal Details


  • Name: Dr. Vikas Jain
  • Designation: Associate Professor
  • Department: Biological Sciences
  • E-Mail: vikas[AT]iiserb.ac.in
  • Phone: +91 755 269 1425, 669 1425


Academic Details


  • Associate Professor (September 2015 - till date), Biological Sciences, IISER Bhopal, India.
  • Assistant Professor (June 2009 - August 2015), Biological Sciences, IISER Bhopal, India.
  • Ph.D. in (Biological Science) 2006 from Molecular Biophysics Unit, Indian Institute of Science, Bangalore.

Research Interest


  • The MMBL group at IISER Bhopal is actively involved in both basic and applied research in bacteriophages. In particular, we are studying the process of phage infection of mycobacteria and the downstream activities that occur inside the cell post-infection. Several proteins that are involved in transcription of phage late genes and the release of mature phage particles are of specific interest in addressing questions related to their regulation and future application in phage-based therapy for tuberculosis infection. Additionally, we are also looking into the bacterial perspective, in terms of tolerance development, resistance to phage infection and evolution of such phenotype. We have developed a combinatorial approach to understand the phage-host system that utilizes both basic and advanced research tools available in Biophysics, Biochemistry, Molecular Biology and Microbiology to address specific questions such as the process of phage infection, phage gene transcription, virion release, bacterial cell injury and recovery, cell death and cell survival strategies. We employ spectroscopic methods, including circular dichroism, steady state and time resolved fluorescence coupled with 2D proteomics and mass spectrometric approaches, as well as biophysical and modern microscopic imaging to gain novel insight into the unique interaction that has evolved between mycobacteriophage and its host.

Research Areas


  • Microbiology
  • Molecular Biology
  • Biochemistry
  • Molecular Biophysics
Authors Title of Article Journal Year
Manika Indrajit Singh
Bylapudi Ganesh
Vikas Jain
On the domains of T4 phage sliding clamp gp45: an intermolecular crosstalk governs structural stability and biological activity. BBA-General Subjects 2017
Radhakrishnan Mahalakshmi
Svetlana Rajkumar Maurya
Bhawna Burdak
Parini Surti
Manoj S Patel
Vikas Jain
Structural plasticity of T4 transcription co-activator gp33 revealed by a protease-resistant unfolded state Biophysical and Biochemical Research Communication 2017
Soumya Kamilla
Vikas Jain
Robust high throughput real-time monitoring assay for the specific screening of bacterial cell envelope inhibitors Proc. Indian Natn. Sci. Acad. 2017
Himanshu Joshi
Vikas Jain
Novel method to rapidly and efficiently lyse Escherichia coli for the isolation of recombinant protein Analytical Biochemistry 2017
Manika Indrajit Singh
Vikas Jain
Identification and characterization of an inside-out folding intermediate of T4 phage sliding clamp Biophysical Journal 2017
Himanshu Joshi
Surya P Seniya
Venkatesan Suryanarayanan
Neelam D Patidar
Sanjeev K Singh
Vikas Jain
Dissecting the structure–function relationship in lysozyme domain of mycobacteriophage D29-encoded peptidoglycan hydrolase FEBS Lett. 2017
Abhishek Anil Dubey
Manika Indrajit Singh
Vikas Jain
Rapid and robust PCR-based all-recombinant cloning methodology PLoS One 2016
Muralikrishna Lella
Soumya Kamilla
Vikas Jain
Radhakrishnan Mahalakshmi
Molecular mechanism of holin transmembrane domain I in pore formation and bacterial cell death ACS Chemical Biology 2016
Manika Indrajit Singh
Vikas Jain
Molecular dissection of the homotrimeric sliding clamp of T4 phage: Two domains of a subunit display asymmetric characteristics Biochemistry 2016
Soumya Kamilla
Vikas Jain
Mycobacteriophage D29 holin C-terminal region functionally assists in holin aggregation and bacterial cell death FEBS Journal 2016
Amol Arunrao Pohane
Vikas Jain
Insights into the regulation of bacteriophage endolysin: Multiple means to the same end Microbiology 2015
Amol Arunrao Pohane
Neelam Devidas Patidar
Vikas Jain
Modulation of domain-domain interaction and protein's function by a charged linker: A case study of mycobacteriophage D29 endolysin. FEBS Lett. 2015
Aashish Sharma
Amol Arunrao Pohane
Sandhya Bansal
Avinash Bajaj
Vikas Jain
Aasheesh Srivastava
Cell Penetrating Synthetic Antimicrobial Peptides (SAMPs) Exhibiting Potent and Selective Killing of Mycobacterium by Targeting Its DNA Chemistry - A European Journal 2015
Kirtimaan Syal
Himanshu Joshi
Dipankar Chatterji
Vikas Jain
Novel pppGpp binding site at the C-terminal region of Rel enzyme from Mycobacterium smegmatis FEBS Journal 2015
Lakhan Ekal
Bylapudi Ganesh
Himanshu Joshi
Dilraj Lama
Vikas Jain
Evidence of a conserved intrinsically disordered region in the C-terminus of the stringent response protein Rel from mycobacteria. FEBS Lett. 2014
Amol Arunrao Pohane
Himanshu Joshi
Vikas Jain
Molecular dissection of phage endolysin: An interdomain interaction confers host specificity in Lysin A of Mycobacterium phage D29. Journal of Biological Chemistry 2014
Manika Indrajit Singh
Vikas Jain
Tagging the Expressed Protein with 6 Histidines: Rapid Cloning of an Amplicon with Three Options. PLoS One 2013
Kelly-Anne F Twist
Elizabeth A Campbell
Padraig Deighan
Sergei Nechaev
Vikas Jain
E Peter Geiduschek
Ann Hochschild
Seth A Darst
Crystal structure of the bacteriophage T4 late-transcription coactivator gp33 with the β-subunit flap domain of Escherichia coli RNA polymerase Proceedings of the National Academy of Sciences 2011

PhD Students


  • Mr. Himanshu Joshi
  • Mr. Abhishek Anil Dubey
  • Mr. Surya Pratap Seniya
  • Mr. Varun Rakeshbhai Bavda
  • Ms. Varsha Patil
  • Mr. Gokul Nair
  • Ms. Rutuja Gangakhedkar
  • Ms. Saloni Wani