Department of Biological Sciences
Indian Institute of Science Education and Research Bhopal


Personal Details


  • Name: Prof. Raghuvir Singh Tomar
  • Designation: Professor
  • Department: Biological Sciences
  • E-Mail: Rst@iiserb.ac.in
  • Phone: +91 755 269 1411


Educational Qualifications


  • B.Sc. : in Biology from Jiwaji University, Gwalior, INDIA, 1991
  • M.Sc. : in Biochemistry from Jiwaji University, Gwalior, INDIA, 1993
  • Ph.D. : Ph.D. in Zoology, 2001 from Center for Advanced Study in Zoology, Banaras Hindu University, Varanasi, INDIA

Post-Doctoral Training


  • May 2002-September 2003: at University of Texas, Southwestern Medical Centre, Dallas (USA).
  • October 2003 - December 2006, at The Penn State University, University Park (USA).
  • January 2007- December 2008, at The Ohio state university, Columbus (USA).

Institutional Responsibilities


  • Coordinator of Department of Biological Sciences, IISER Bhopal: 2010 - 2012
  • Head, Department of Biological sciences, IISER Bhopal: January, 2015 - March, 2018
  • Dean, Student affairs, IISER Bhopal: August, 2018 onward

Vacancies


  • If you are interested in learning fundamentals of chromatin biology, role of epigenetics in stress response, to work with Saccharomyces cerevisiae and chicken, please contact:

    Dr. Raghuvir Singh Tomar
    Laborator of Chromatin Biology, Department of Biological Sciences
    Indian Institute of Science Education& Research Bhopal
    Email: rst@iiserb.ac.in

To identify the role of epigenetics in maintenance of cellular homeostatic pathways and stress:
In eukaryotes, DNA is organized in the form of chromatin which consists of repeating units called nucleosomes. The structure and function of chromatin is regulated by the specific enzymes that modify DNA and histone proteins to maintain cellular homeostatic mechanisms under a variety of pathophysiological conditions. Several classes of enzymes compete for binding to the chromatin. The coordination among chromatin modifying enzymes is critical for survival under various stress conditions. The imbalance in the activity of chromatin modifying enzymes leads to the development of disease pathologies. We trying to understand the significance of chromatin associated epigenetic changes in response to intra and extracellular factors. Following are our key research questions:


  • To dissect the role of yeast Cell Wall Integrity (CWI) pathway in flocculation of yeast.
  • To investigate the mechanism and significance of proteolytic clipping of histone proteins during development, aging, immunity and sepsis.
  • To study role of core histones, chromatin remodelling complexes in regulation of cellular homeostatic pathways, cell wall integrity and metal homeostasis.
  • To understand the mechanism of action of potential anti-cancer molecules.
Authors Title of Article Journal Year
Shraddha Chauhan
Raghuvir Singh Tomar
Unveiling the molecular networks underlying cellular impairment in Saccharomyces cerevisiae: investigating the effects of magnesium oxide.............. Environmental Science and Pollution Research 2024
Ramesh Kumawat
Raghuvir Singh Tomar
Dissecting the role of mitogen?activated protein kinase Hog1 in yeast flocculation FEBS Journal 2024
Nitu Saha
Raghuvir Singh Tomar
Cdc73 majorly regulates apoptosis-inducing factor (AIF1) in Saccharomyces cerevisiae via the H3K36 methylation FEBS Letters 2024
Nitu Saha
Swati Swagatika
Raghuvir Singh Tomar
Investigation of the acetic acid stress response in Saccharomyces cerevisiae with mutated H3 residues Microbial Cell 2023
Ramesh Kumawat
Raghuvir Singh Tomar
Heavy metal exposure induces Yap1 and Hac1 mediated derepression of GSH1 and KAR2 by Tup1-Cyc8 complex Journal of Hazardous Materials 2022
Nitu Saha
Raghuvir Singh Tomar
Copper inhibits protein maturation in the secretory pathway by targeting the Sec61 translocon in Saccharomyces cerevisiae Journal of Biological Chemistry 2022
Sakshi Singh
Rakesh Kumar Sahu
Anaswara Sugathan
Raghuvir Singh Tomar
The H2A N-terminal tail is required to alleviate copper-induced stress in Saccharomyces cerevisiae FEMS Yeast Research 2021
Swati Swagatika
Raghuvir Singh Tomar
Cantharidin downregulates PSD1 expression and inhibits autophagic flux in yeast cells FEBS Open Biol 2021
Pilendra Kumar Thakre
Rakesh Kumar Sahu
Raghuvir Singh Tomar
Substitution of histone H3 arginine 72 to alanine leads to deregulation of isoleucine biosynthesis in budding yeast Saccharomyces cerevisiae Biochemistry and Cell Biology 2021
Rakesh Kumar Sahu
Sakshi Singh
Raghuvir Singh Tomar
The ATP-dependent SWI/SNF and RSC chromatin remodelers cooperatively induce unfolded protein response genes during endoplasmic reticulum stress BBA-Gene Regulatory Mechanisms 2021
Sakshi Singh
Rakesh Kumar Sahu
Raghuvir Singh Tomar
The N-Terminal Tail of Histone H3 Regulates Copper Homeostasis in Saccharomyces cerevisiae Molecular and Cellular Biology 2021
Swati Swagatika
Raghuvir Singh Tomar
ABC transporter Pdr5 is required for cantharidin resistance in Saccharomyces cerevisiae Biochemical and Biophysical Research Communications 2021
Rakesh Sahu
Sakshi Singh
Raghuvir Tomar
The mechanisms of action of chromatin remodelers and implications in development and diseases Biochemical Pharmacology 2020
Rakesh Sahu
Nitu Saha
Laxmidhar Das
Pushpendra Sahu
Santhosh Sariki
Raghuvir Tomar
SWI/SNF chromatin remodeling complex contributes to clearance of cytoplasmic protein aggregates and regulates unfolded protein response in Saccharomyces cerevisiae FEBS Journal 2020
Pilendra Kumar Thakre
Upendarrao Golla
Ashis Biswas
Raghuvir Singh Tomar
Identification of histone H3 and H4 amino acid residues important for the regulation of arsenite stress signaling in Saccharomyces cerevisiae Chemical Research in Toxicology 2020
Santhosh Kumar Sariki
Ramesh Kumawat
Vikash Singh
Raghuvir Singh Tomar
Flocculation of Saccharomyces cerevisiae is dependent on activation of Slt2 and Rlm1 regulated by the Cell Wall Integrity pathway Molecular Microbiology 2019
Pushpendra Kumar Sahu
Sagar Salin
Sakshi Chauhan
Reverse genetic analysis of yeast YPR099C/MRPL51 reveals a critical role of both overlapping ORFs in respiratory growth and MRPL51 in mitochondrial DNA maintenance FEMS Yeast Research 2019
Pushpendra Kumar Sahu
Raghuvir Singh Tomar
The natural anticancer agent cantharidin alters GPI-anchored protein sorting by targeting Cdc1-mediated remodeling in endoplasmic reticulum Journal of Biological Chemistry 2019
Pushpendra Kumar Sahu
Sakshi Chauhan
Raghuvir Singh Tomar
Crg1 N-terminus is essential for methyltransferase activity and Cantharidin resistance in Saccharomyces cerevisiae Biochemistry 2019
Pilendra Thakre
Athira SV
Upendarrao Golla
Sakshi Chauhan
Raghuvir Tomar
Previously uncharacterized amino acid residues in histone H3 and H4 mutants with roles in DNA damage repair response and cellular aging FEBS Journal 2018
Gajendra Kumar Azad
Modifying Chromatin by Histone Tail Clipping Journal of Molecular Biology 2018
Piyoosh Kumar Babele
Pilendra Kumar Thakre
Ramesh Kumawat
Raghuvir Singh Tomar
Zinc oxide nanoparticles induce toxicity by affecting cell wall integrity pathway, mitochondrial function and lipid homeostasis in Saccharomyces cerevisiae Chemosphere 2018
Biological Sciences SK
Bahadur Kumar Thakre
Pilendra Singh Tomar
Raghuvir Patra
A Pyridoindole-Based Multifunctional Bioprobe: pH-Induced Fluorescence Switching and Specific Targeting of Lipid Droplets Chemistry -Asian Journal 2017
Upendarrao Golla
Swati Swagatika
Sakshi Chauhan
Raghuvir S Tomar
A systematic assessment of chemical, genetic, and epigenetic factors influencing the activity of anticancer drug KP1019 (FFC14A) Oncotarget 2017
Sakshi Chauhan
Mandal Papita
Raghuvir Singh T
The biochemical analysis reveals multifactorial mechanism of histone H3 clipping by Chicken Liver Histone H3 protease Biochemistry 2016
Gajendra Azad
Partial purification of histone H3 proteolytic activity from budding yeast Saccharomyces cerevisiae Yeast 2016
Sakshi Chauhan
Efficient expression and purification of biologically active human cystatin proteins Protein Expression and Purification 2016
Santhosh Kumar Sariki
Pushpendra Kumar Sahu
Upendarao Golla
Gajendra Kumar Azad
Vikash
Raghuvir Singh T
Sen1, the homolog of human Senataxin, is critical for survival through regulation of cellular redox homeostasis, mitochondrial function and TOR in Saccharomyces cerevisiae FEBS Journal 2016
Upendarrao Golla
Deepthi Joseph
Raghvir Singh Tomar
Combined Transcriptomics and Chemical-Genetics Reveal Molecular Mode of Action of Valproic acid, an Anticancer Molecule using Budding Yeast Model Scientific Reports 2016
Gajendra Kumar Azad
Raghuvir Singh Tomar
The multifunctional transcription factor Rap1: a regulator of yeast physiology Frontiers Bioscience 2016
Upendarrao Golla
Amit Adhikary
Amit Kumar Mondal
Raghuvir Singh Tomar
Sanjit Konar
Synthesis, structure, magnetic and biological activity studies of bis-hydrazone derived Cu(II) and Co(II) coordination compounds Dalton Transactions 2016
Gajendra Kumar Azad
Vikash Singh
Shivani Barnawal
Mayur J Thakare
Raghuvir Singh Tomar
The transcription factor Rap1p is required for tolerance to cell wall-perturbing agents and for cell wall maintenance in Saccharomyces cerevisiae FEBS Lett. 2015
Vikash Singh
Gajendra Kumar Azad
Santhosh Kumar Sariki
Raghuvir Singh Tomar
Flocculation in Saccharomyces cerevisiae is regulated by RNA/DNA helicase Sen1p FEBS Lett. 2015
Upendarrao Golla
Bandi Goutham
Raghuvir Singh Tomar
Molecular Cytotoxicity Mechanisms of the Herbicide Allyl Alcohol (Acrolein) in Budding Yeast Chemical Research in Toxicology 2015
Gajendra Kumar Azad
Vikash Singh
Raghuvir Singh Tomar
Assessment of the biological pathways targeted by isocyanate using N-succinimidyl N-methylcarbamate on budding yeast Saccharomyces cerevisiae. PLoS One 2014
Vikash Singh
Gajendra Kumar Azad
Papita Mandal
Amrendar M Reddy
Shivani Baranwal
Raghuvir Singh Tomar
Anti-cancer drug KP1019 induces Hog1 phosphorylation and protein ubiquitination in Saccharomyces cerevisiae Eur J. Pharmacology 2014
Vikash Singh
Gajendra Kumar Azad
Papita Mandal
Amrendar M Reddy
Raghuvir Singh Tomar
Anti-cancer drug KP1019 modulates epigenetics and induces DNA damage response in Saccharomyces cerevisiae FEBS Lett. 2014
Shivani Barnawal
Gajendra Kumar Azad
Vikash Singh
Raghuvir Singh Tomar
Signaling of chloroquine-induced stress in the yeast Saccharomyces cerevisiae requires the Hog1 and Slt2 MAPK Pathways Antimicrobial Agents and Chemotherapy 2014
Papita Mandal
Sakshi Chauhan
Raghuvir Tomar
H3 clipping activity of glutamate dehydrogenase is regulated by stefin B and chromatin structure FEBS Journal 2014
Gajendra Kumar Azad
Vikash Singh
Mayur J Thakare
Shivani Barnawal
Raghuvir Singh Tomar
Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae BMC Microbiology 2014
Gajendra Kumar Azad
Vikash Singh
Papita Mandal
Prabhat Singh
Upendarrao Golla
Sakshi Chauhan
Shivani Barnawal
Raghuvir Singh Tomar
Ebselen induces reactive oxygen species (ROS)-mediated cytotoxicity in Saccharomyces cerevisiae with inhibition of glutamate dehydrogenase being a target FEBS Open Biol 2014
Papita Mandal
Naveen Verma
Gajendra Kumar Azad
Vikash Singh
Upendarrao Golla
Raghuvir Singh Tomar
Epigenetics: Role of histone proteases in cellular functions and diseases. A book chapter in ‘Molecular Mechanisms and Physiology of Disease: Implications for Epigenetics and Health’. Maulik, N.; Karagiannis, T. (Eds.) Springer press 2014
Gajendra Kumar Azad
Raghuvir Singh Tomar
The emerging role of histone proteases in regulation of various biological processes Molecular Biology Reports 2014
Gajendra Kumar Azad
Raghuvir Singh Tomar
A comprehensive review illustrating the mode of action and biological pathways targeted by promising antioxidant drug, ebselen Molecular Biology Reports 2014
Papita Mandal
Naveen Verma
Sakshi Chauhan
Raghuvir Singh Tomar
Unexpected histone H3 tail clipping activity of Glutamate dehydrogenase Journal of Biological Chemistry 2013
Upendarrao Golla
Vikash Singh
Gajendra Kumar Azad
Prabhat Singh
Papita Mandal
Raghuvir Singh Tomar
Sen1p contributes to genomic integrity by regulating expression of Ribonucleotide reductase 1 (RNR1) in Saccharomyces cerevisiae PLoS One 2013
Gajendra Kumar Azad
Vikash Singh
Upendarrao Golla
Raghuvir Singh Tomar
Depletion of cellular iron by curcumin leads to alteration in histone acetylation and degradation of Sml1p in Saccharomyces cerevisiae. PLoS One 2013
Papita Mandal
Gajendra Kumar Azad
Raghuvir Singh Tomar
Identification of a novel histone H3 specific protease activity in nuclei of chicken liver Biophysical and Biochemical Research Communication 2012
Gajendra Kumar Azad
Shah Jaimin Balkrishna
Narayanan Sathish
Sangit Kumar
Raghuvir Singh Tomar
Multifunctional Ebselen drug functions through the activation of DNA damage response and alterations in nuclear proteins Biochemical Pharmacology 2012

PhD Students


  • Ramesh Kumawat, 1510110
  • Rakesh Kumar Sahu, 1610109
  • Nitu Saha, 1610105
  • Sakshi Singh, 1620107
  • Ranu Singh, 1820114
  • Santoshi Acharjee

BS-MS Student


  • Srobona Mahajan
  • Aryama Das

Ph.D. students graduated


  • Dr. Papita Mandal, Post-Doctoral Fellow at King Abdullah University Science Technology, Saudi Arabia.
  • Dr. Gajendra Kumar Azad, Assistant Professor, department of Zoology, University of Patna.
  • Dr. Vikash Singh, Post-doctoral fellow at Penn State University, USA.
  • Dr. Sakshi Chauhan, Post-doctoral fellow at NIH, USA
  • Dr. Upendarrao Golla, Post-doctoral fellow at Penn State University, USA.
  • Dr. Pushpendra Kumar Sahu, Post-doctoral fellow at Max Planck, Germany.
  • Dr. Pilendra Kumar Thakre, Post-doctoral fellow, joining at Baylor college of Medicine, USA
  • Dr. Santhosh Kumar Sariki, 1520105, Post-doctoral fellow at University of Michigan, USA
  • Dr. Swati Swagatika