Department of Biological Sciences
Indian Institute of Science Education and Research Bhopal


Personal Details


  • Name: Dr. Raghuvir Singh Tomar
  • Designation: Professor
  • Department: Biological Sciences
  • E-Mail: rst[AT]iiserb.ac.in
  • Phone: +91 755 269 1411


Academic Details


  • Professor (February, 2018- Present), Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India.
  • Associate Professor (November, 2013- January, 2018), Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India.
  • Assistant Professor (January, 2009- November, 2013), Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India.
  • Post-doc. January 2007- December 2008, The Ohio State University, Columbus (USA).
  • Post-doc. October 2003 - December 2006, The Penn State University, University Park (USA).
  • Post-doc. May 2002 - September 2003, University of Texas, Southwestern Medical Centre, Dallas (USA).

Current News


  • Papita Mandal got selected for prestigious ‘Kestone Symposia scholarship’ funded by the German Ministry of Education and Research to present a poster in the Keystone symposia meeting ‘Chromatin Mechanisms and Cell Physiology’ at Oberstdorf, Germany, March 23 – 28, 2014
  • Best poster award to Papita Mandal in IISERB Biology meeting, January 11 – 12, 2014
  • Best oral presentation award to Gajendra K. Azad in IISERB Biology meeting, January 1 – 12, 2014
  • Travel award to Gajendra K. Azad from Department of Science and Technology, Govt. of India for presenting a poster in Wellcome Trust meeting Chromatin: from nucleosomes to chromosomes' at Wellcome Trust Genome Campus, Hinxton, Cambridge, UK, April 30 May 2, 2014
  • Gajendra delivered Open Ph.D. seminar on May 30th.
  • Papita delivered open Ph.D. seminar on June 6th.
  • Gajendra got selected for post doc position at Hebrew University of Jerusalem, Israel in Dr. Eran Meshorer lab
  • Papita got selected for post doc position at Max Planck Institute of Biological Chemistry, Gottingen, Germany in Dr. Wolfgang Fischle lab.
  • Travel award to Vikash from DST to present his work in 'Chromatin and Transcription' meeting held at EMBL, Heidelberg, Germany from 23 - 26 August, 2014.
  • Congratulations to Papita for successful thesis defence held on October 3rd, 2014.
  • Congratulations to Gajendra for successful completion of thesis defence held on November 20, 2014.

Vacancies


  • Vacancies are available for Junior Research and Post-doctoral fellows. Interested candidates having experience in epigenetics, proteomics, gene expression, mass spectrometry, microscopy and cell culture may contact through email. Shortlisted applicants will be called for interview.

    Contact:
    Dr. Raghuvir S. Tomar
    Associate Professor
    Laborator of Chromatin Biology, Department of Biological Sciences
    Indian Institute of Science Education& Research Bhopal
    Email: rst@iiserb.ac.in

To study the role of chromatin dependent mechanisms in response to genetic and epigenetic perturbations


In eukaryotes DNA is compacted in the form of chromatin which consists of repeating units called nucleosomes; composed of 146 bp of DNA and a histone octamer. Structure of chromatin is dynamically regulated by the specific enzymes that modify DNA and histone proteins in reversible manner. Dynamic structure of chromatin is the fundamental underlying mechanism for gene expression. Several classes of enzymatic regulators that compete for binding to the chromatin dictate the outcome and the coordination among them to maintain a fluid chromatin structure at a particular locus for normal gene expression is unclear. Imbalance in the activity or expression of chromatin regulators may lead to the development of diseases. Currently in my lab three main research problems are under investigations to understand the significance of chromatin associated epigenetic changes in response to intra and extracellular factors:


  • To identify the role of yeast repressor activator protein (Rap1) in chromatin remodelling of DNA response and Cell Wall Integrity (CWI) pathway genes: Rap1 is a telomere binding protein primarily known for transcription regulation of glycolytic and ribosomal protein genes. Recently it has been shown that Rap1 binds with components of transcription machinery to activate the transcription of Ribonucleotide Reductase (RNR) genes. Through chromatin chip experiment Rap1 protein has been found cross linked with promoter of RNR genes under repressed and activated states. Two classes of chromatin remodelling complexes; ISW2 and SWI/SNF2 are involved in repression and activation of transcription respectively. Rap1 mutant yeast cells are sensitive to DNA and Cell wall damaging agents. How does Rap1 cooperate in vivo with these two opposing actions of remodelling complexes and histones is unclear. We are addressing these questions by employing the combination of in vitro biochemical and genetic approaches.
  • To investigate the mechanism and physiological significance of proteolytic clipping of histone H3: In several organisms including humans, yeast and Xenopus, nucleosomal Histone H3 has been found proteolytically clipped. While the role of reversible histone modifications is well established, very little is known about the proteolytic clipping of histones which has also been linked in regulation of gene expression. In many cases the identity of the protease is also known. Using yeast and chicken as model organisms we are trying to study the biological significance of this epigenetic event during development and aging.
  • Role of epigenetics in drug metabolism: This is one of the important topics of research but poorly understood. It has great potential in modern medicine. Histone proteins that package DNA plays important regulatory role in replication, transcription, DNA repair and recombination. Several combinations of post-translational modifications of the histone proteins which is known as ‘histone code’, influences the expression of genes whose products are engaged in drug metabolism. We are trying to understand the role of epigenetic alterations and gene expression in response to pharmacologically important drugs.





Key research objectives:

  • To study cellular signalling pathways in response to genetic and epigenetic perturbations.
  • To study role of core histones and chromatin remodelling complexes in genomic integrity and cell wall integrity, metal homeostasis and anti-cancer molecules.
  • To study physiological significance to proteolytic clipping of core histones.
Authors Title of Article Journal Year
Pilendra Thakre
Athira SV
Upendarrao Golla
Sakshi Chauhan
Raghuvir Tomar
Previously uncharacterized amino acid residues in histone H3 and H4 mutants with roles in DNA damage repair response and cellular aging FEBS Journal 2018
Gajendra Kumar Azad
Modifying Chromatin by Histone Tail Clipping Journal of Molecular Biology 2018
Piyoosh Kumar Babele
Pilendra Kumar Thakre
Ramesh Kumawat
Raghuvir Singh Tomar
Zinc oxide nanoparticles induce toxicity by affecting cell wall integrity pathway, mitochondrial function and lipid homeostasis in Saccharomyces cerevisiae Chemosphere 2018
Biological Sciences SK
Bahadur Kumar Thakre
Pilendra Singh Tomar
Raghuvir Patra
A Pyridoindole-Based Multifunctional Bioprobe: pH-Induced Fluorescence Switching and Specific Targeting of Lipid Droplets Chemistry -Asian Journal 2017
Upendarrao Golla
Swati Swagatika
Sakshi Chauhan
Raghuvir S Tomar
A systematic assessment of chemical, genetic, and epigenetic factors influencing the activity of anticancer drug KP1019 (FFC14A) Oncotarget 2017
Sakshi Chauhan
Mandal Papita
Raghuvir Singh T
The biochemical analysis reveals multifactorial mechanism of histone H3 clipping by Chicken Liver Histone H3 protease Biochemistry 2016
Gajendra Azad
Partial purification of histone H3 proteolytic activity from budding yeast Saccharomyces cerevisiae Yeast 2016
Sakshi Chauhan
Efficient expression and purification of biologically active human cystatin proteins Protein Expression and Purification 2016
Santhosh Kumar Sariki
Pushpendra Kumar Sahu
Upendarao Golla
Gajendra Kumar Azad
Vikash
Raghuvir Singh T
Sen1, the homolog of human Senataxin, is critical for survival through regulation of cellular redox homeostasis, mitochondrial function and TOR in Saccharomyces cerevisiae FEBS Journal 2016
Upendarrao Golla
Deepthi Joseph
Raghvir Singh Tomar
Combined Transcriptomics and Chemical-Genetics Reveal Molecular Mode of Action of Valproic acid, an Anticancer Molecule using Budding Yeast Model Scientific Reports 2016
Gajendra Kumar Azad
Raghuvir Singh Tomar
The multifunctional transcription factor Rap1: a regulator of yeast physiology Frontiers Bioscience 2016
Upendarrao Golla
Amit Adhikary
Amit Kumar Mondal
Raghuvir Singh Tomar
Sanjit Konar
Synthesis, structure, magnetic and biological activity studies of bis-hydrazone derived Cu(II) and Co(II) coordination compounds Dalton Transactions 2016
Gajendra Kumar Azad
Vikash Singh
Shivani Barnawal
Mayur J Thakare
Raghuvir Singh Tomar
The transcription factor Rap1p is required for tolerance to cell wall-perturbing agents and for cell wall maintenance in Saccharomyces cerevisiae FEBS Lett. 2015
Vikash Singh
Gajendra Kumar Azad
Santhosh Kumar Sariki
Raghuvir Singh Tomar
Flocculation in Saccharomyces cerevisiae is regulated by RNA/DNA helicase Sen1p FEBS Lett. 2015
Upendarrao Golla
Bandi Goutham
Raghuvir Singh Tomar
Molecular Cytotoxicity Mechanisms of the Herbicide Allyl Alcohol (Acrolein) in Budding Yeast Chemical Research in Toxicology 2015
Gajendra Kumar Azad
Vikash Singh
Raghuvir Singh Tomar
Assessment of the biological pathways targeted by isocyanate using N-succinimidyl N-methylcarbamate on budding yeast Saccharomyces cerevisiae. PLoS One 2014
Vikash Singh
Gajendra Kumar Azad
Papita Mandal
Amrendar M Reddy
Shivani Baranwal
Raghuvir Singh Tomar
Anti-cancer drug KP1019 induces Hog1 phosphorylation and protein ubiquitination in Saccharomyces cerevisiae Eur J. Pharmacology 2014
Vikash Singh
Gajendra Kumar Azad
Papita Mandal
Amrendar M Reddy
Raghuvir Singh Tomar
Anti-cancer drug KP1019 modulates epigenetics and induces DNA damage response in Saccharomyces cerevisiae FEBS Lett. 2014
Shivani Barnawal
Gajendra Kumar Azad
Vikash Singh
Raghuvir Singh Tomar
Signaling of chloroquine-induced stress in the yeast Saccharomyces cerevisiae requires the Hog1 and Slt2 MAPK Pathways Antimicrobial Agents and Chemotherapy 2014
Papita Mandal
Sakshi Chauhan
Raghuvir Tomar
H3 clipping activity of glutamate dehydrogenase is regulated by stefin B and chromatin structure FEBS Journal 2014
Gajendra Kumar Azad
Vikash Singh
Mayur J Thakare
Shivani Barnawal
Raghuvir Singh Tomar
Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae BMC Microbiology 2014
Gajendra Kumar Azad
Vikash Singh
Papita Mandal
Prabhat Singh
Upendarrao Golla
Sakshi Chauhan
Shivani Barnawal
Raghuvir Singh Tomar
Ebselen induces reactive oxygen species (ROS)-mediated cytotoxicity in Saccharomyces cerevisiae with inhibition of glutamate dehydrogenase being a target FEBS Open Biol 2014
Papita Mandal
Naveen Verma
Gajendra Kumar Azad
Vikash Singh
Upendarrao Golla
Raghuvir Singh Tomar
Epigenetics: Role of histone proteases in cellular functions and diseases. A book chapter in ‘Molecular Mechanisms and Physiology of Disease: Implications for Epigenetics and Health’. Maulik, N.; Karagiannis, T. (Eds.) Springer press 2014
Gajendra Kumar Azad
Raghuvir Singh Tomar
The emerging role of histone proteases in regulation of various biological processes Molecular Biology Reports 2014
Gajendra Kumar Azad
Raghuvir Singh Tomar
A comprehensive review illustrating the mode of action and biological pathways targeted by promising antioxidant drug, ebselen Molecular Biology Reports 2014
Papita Mandal
Naveen Verma
Sakshi Chauhan
Raghuvir Singh Tomar
Unexpected histone H3 tail clipping activity of Glutamate dehydrogenase Journal of Biological Chemistry 2013
Upendarrao Golla
Vikash Singh
Gajendra Kumar Azad
Prabhat Singh
Papita Mandal
Raghuvir Singh Tomar
Sen1p contributes to genomic integrity by regulating expression of Ribonucleotide reductase 1 (RNR1) in Saccharomyces cerevisiae PLoS One 2013
Gajendra Kumar Azad
Vikash Singh
Upendarrao Golla
Raghuvir Singh Tomar
Depletion of cellular iron by curcumin leads to alteration in histone acetylation and degradation of Sml1p in Saccharomyces cerevisiae. PLoS One 2013
Papita Mandal
Gajendra Kumar Azad
Raghuvir Singh Tomar
Identification of a novel histone H3 specific protease activity in nuclei of chicken liver Biophysical and Biochemical Research Communication 2012
Gajendra Kumar Azad
Shah Jaimin Balkrishna
Narayanan Sathish
Sangit Kumar
Raghuvir Singh Tomar
Multifunctional Ebselen drug functions through the activation of DNA damage response and alterations in nuclear proteins Biochemical Pharmacology 2012

PhD Students


  • Dr. Sakshi Chauhan, Graduated in 2017
  • Dr. Upendarrao Golla, Graduated in 2017
  • Pushpendra Kumar Sahu, Registered for Ph.D. in August, 2014
  • Pilendra Kumar Thakre, Registered for Ph.D. in January, 2015
  • Swati Swagatika, Registered for Ph.D. in August 2015
  • Ramesh Kumawat, Registered for Ph.D. in August, 2015
  • Santhosh Kumar Sariki, Registered for Ph.D. in January, 2016
  • Rakesh Kumar Sahu, Registered for Ph.D. in August, 2016
  • Nitu Saha, Registered for Ph.D. in August, 2016
  • Sakshi Singh, Registered for Ph.D. in January, 2017

Post-Doctoral Fellow


  • Dr. Piyoosh Kumar Babele, SERB N-PDF

BS-MS Student


  • Mahajan Vibhuti Prasad, BS-MS project
  • Rohit Kumar, BS-MS project

Summer Trainees


  • Oza Yagna Anand
  • Asawari Alwal

PhD Awarded


  • Dr. Papita Mandal, joined as Post-Doctoral Fellow at King Abdullah University Science Technology, Saudi Arabia.
  • Dr. Gajendra Kumar Azad, joined as Post-Doctoral Fellow at The Hebrew University of Jerusalem, Israel.
  • Dr. Vikash Singh, joined as Post-Doctoral Fellow at Penn State University, Hershey Medical Centre, United States.
  • Dr. Sakshi Chauhan,
  • Dr. Upendarrao Golla

BS-MS Awarded


  • Naveen Verma, BS-MS 2013. Joined University of Utah, United States for Ph.D.
  • Goutham Bandi, BS-MS 2014
  • Jagriti Banerjee, BS-MS 2015
  • Rishith Ravindran, BS-MS 2015, joined IISER TVM for Ph.D.
  • Deepthi Joseph, BS-MS 2015, Joined NCBS for Ph.D.
  • Mubthasima PP, BS-MS 2016
  • Jeena TJ, BS-MS 2016
  • Sagar Salim, BS-MS 2017
  • Athira SV, BS-MS 2017
  • Soumya Prakash Sahu, BS-MS 2017